X-Gal, 40mg/ml in DMF (5-Bromo-4-chloro-3-indolyl-b-D-galactoside galactopyranoside, Xgal)

Blue substrate used in the detection of b-galactosidase in bacteria or phage as a selection agent for cloning experiments utilizing the lacZ vector. Colonies expressing b-galactosidase will appear blue in the presence of XGAL. Others will appear white.\n\nPurity (HPLC): (same/more than)99%\nPurity (TLC): Single Spot\nConcentration: ~40mg/ml\nForm: Supplied as a liquid in dimethylformamide.\nSolubility: Colorless to pale yellow, clear, complete\nIdentity by I.R. or NMR: Conforms to structure\n\nRNase, DNase or Proteases: None Detected\n\nQuality Control (Blue-White Assay):\n[LacZ+]: Pale blue center/dense blue periphery\n[LacZ-]: Faint blue center/white periphery\n\nImportant Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.\n\nStorage and Stability:\nStore at -20 degrees C. Aliquots are stable for 12 months. Protect from light.\n\nAlso Available:\nX1000: XGAL\nX1000-05: XGAL, 40mg/ml in DMF

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

X1000-05

Size

10ml

Form

Supplied as a liquid in dimethylformamide.

References

1. Horvitz, J.P., et al., J. Med. Chem. 7:574 (1964). 2. Lin, W.C., et al., Bacterial lacZ gene as a highly sensitive marker to detect micrometastasis formation during tumor progression. Cancer Res. 50:2808-2817 (1990). 3. Buckner, F.S., et al., Detection of live Trypanosoma cruzi in tissues of infected mice by using histochemical stain for beta-galactosidase. Infect. Immun. 67:403-409 (1999) 4. Weiss, D.J., et al., In situ histochemical detection of beta-galactosidase activity in lung. Hum. Gene Ther. 8:1545-1554 (1997). 5. Sambrook, J., et al., Molecular Cloning: A Laboratory Manual , Plainview, NY (1989), 1.85-1.86 and B.14.

Alternative Names

5-Bromo-4-chloro-3-indolyl-b-D-galactoside (galactopyranoside) in Dimethylformamide