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X-Gal, 40mg/ml in DMF (5-Bromo-4-chloro-3-indolyl-b-D-galactoside galactopyranoside, Xgal)

X-Gal, 40mg/ml in DMF (5-Bromo-4-chloro-3-indolyl-b-D-galactoside galactopyranoside, Xgal)

Cat no: X1000-05

Supplier: United States Biological
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Blue substrate used in the detection of b-galactosidase in bacteria or phage as a selection agent for cloning experiments utilizing the lacZ vector. Colonies expressing b-galactosidase will appear blue in the presence of XGAL. Others will appear white. Purity (HPLC): (same/more than)99% Purity (TLC): Single Spot Concentration: ~40mg/ml Form: Supplied as a liquid in dimethylformamide. Solubility: Colorless to pale yellow, clear, complete Identity by I.R. or NMR: Conforms to structure RNase, DNase or Proteases: None Detected Quality Control (Blue-White Assay): [LacZ+]: Pale blue center/dense blue periphery [LacZ-]: Faint blue center/white periphery Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Storage and Stability: Store at -20 degrees C. Aliquots are stable for 12 months. Protect from light. Also Available: X1000: XGAL X1000-05: XGAL, 40mg/ml in DMF
Catalogue number: X1000-05
Size: 10ml
Form: Supplied as a liquid in dimethylformamide.
Alternative names: 5-Bromo-4-chloro-3-indolyl-b-D-galactoside (galactopyranoside) in Dimethylformamide
References: 1. Horvitz, J.P., et al., J. Med. Chem. 7:574 (1964). 2. Lin, W.C., et al., Bacterial lacZ gene as a highly sensitive marker to detect micrometastasis formation during tumor progression. Cancer Res. 50:2808-2817 (1990). 3. Buckner, F.S., et al., Detection of live Trypanosoma cruzi in tissues of infected mice by using histochemical stain for beta-galactosidase. Infect. Immun. 67:403-409 (1999) 4. Weiss, D.J., et al., In situ histochemical detection of beta-galactosidase activity in lung. Hum. Gene Ther. 8:1545-1554 (1997). 5. Sambrook, J., et al., Molecular Cloning: A Laboratory Manual , Plainview, NY (1989), 1.85-1.86 and B.14.

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