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X-Gal (5-Bromo-4-chloro-3-indolyl-b-D-galactoside galactopyranoside, Xgal)

X-Gal (5-Bromo-4-chloro-3-indolyl-b-D-galactoside galactopyranoside, Xgal)

Cat no: X1000

Supplier: United States Biological
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Blue substrate used in the detection of b-galactosidase in bacteria or phage as a selection agent for cloning experiments utilizing the lacZ vector. Colonies expressing b-galactosidase will appear blue in the presence of XGAL. Others will appear white. Purity (HPLC): (same/more than)99% Purity (TLC): Single Spot Appearance: White lyophilized powder Solubility: Soluble in DMF Water (KF): (same/less than)1% Specific Rotation: -60 to -64 Identity by I.R. or NMR: Conforms to structure RNase, DNase or Proteases: None Detected Quality Control (Blue-White Assay): [LacZ+]: Pale blue center/dense blue periphery [LacZ-]: Faint blue center/white periphery Storage and Stability: Store at -20 degrees C. Stable for 6 months. Protect from light. Also Available: X1000: XGAL X1000-05: XGAL, 40mg/ml in DMF
Catalogue number: X1000
Size: 100mg
Alternative names: (X-Gal, 5-Bromo-4-chloro-3-indolyl-b-D-galactoside (galactopyranoside))
References: US Biological application references: 1. Hwang, L.H and Murray, A.W. (1997) Mol. Biol. Cell 8:1877-1887. 2. Tsuchida, T. et al., (2004) Biotechniques 37:896-897. 3. Coombs, J.M and Brenchley, J.E. (1999) Applied and Environmental Microbiology, 65:5443-5450. 4. Itkin-Ansari, P. et al., (2005) Developmental Dynamics 233:946

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