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X-Gluc Cyclohexylammonium Salt (XGLUC)

X-Gluc Cyclohexylammonium Salt (XGLUC)

Cat no: X1025

Supplier: United States Biological
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Indigo-blue chromogenic substrate used in the detection of beta-glucuronidase (GUS fusion marker) in E. coli for transcription and translation studies. Additional applications include bacterial contamination testing in water, meat, dairy, shellfish. The chromogenic substrate X-Gluc is used in a variety of applications for the detection of the B-glucuronidase enzyme. Upon reduction, X-Gluc produces a localized color, making it useful in identifying GUS gene presence in most cell types1 and for the detection of the GUS gene fusion marker in plants. X-Gluc has reported applications in the detection of contaminated food samples such as meat, dairy, and shellfish products. X-Gluc also has clinical applications in the assessment of urinary tract infection by d etecting the pr esence of E. coli. It has gained international acceptance as an accurate indicator for the presence of E. coli in potable water samples b y reducing false positives and negatives found with traditional methods. Solubility (1% DMF/water 1/1): Colorless, clear, complete Water (KF): (same/less than)5.0% Storage and Stability: Store at -20C under nitrogen. Protect from light and humidity.
Catalogue number: X1025
Size: 100mg
Form: Whute needles
Purity: (same/more than)99%
Alternative names: 5-Bromo-4-chloro-3-indolyl-b-D-glucuronide cyclohexylammonium salt
References: 1. Ellis, D.D., et al., Bio/Technology, 11(1): 84-89 (1993). 2. Bommineni, V.R., et al., Plant Cell Reports, 13(1): 17-23 (1993). 3. Martin, G.C., et al., J. Amer. Soc. Horticult. Sci., 115(46): 686-691 (1990). 4. Restaino, L., et al., J. Food Protect., 53(6): 508-510 (1990). 5. Watkins, W.D., et al., Appl. Environ. Microbiol., 54: 1874-1875 (1988). 6. Delisle, G.J., and Ley, A., J. Clin. Microbiol. 27(4): 778-779 (1989). 7. Gaudet, I.D., et al., Appl. Environ. Microbiol., 62(11): 4032-4035 (1996). 8. Ceibin, B.W., et al., Appl. Environ. Microbiol., 61(11): 3940-3942 (1995). 9. Sartory, D.P., and Howard, L., Letters in Applied Microbiology, 15: 2373-2376 (1992). 10. Brenner, K.P., et al., Abstracts for the Annual Meeting of the American Society of Microbiology (1993). 11. Frampton, E.W., et al., J. Food Protect. 51(5): 402-404 (1988). 12. Van Pouckle, S.O., and Nelis, H.J., Appl. Environ. Microbiol., 63(2): 771-774 (1997).

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