X-prolyl aminopeptidase (aminopeptidase P, 2 Aminoacylproline aminopeptidase, mAmP, Membrane-bound aminopeptidase P, Membrane-bound AmP, Membrane-bound APP, Xaa-Pro aminopeptidase 2 precursor, X-Pro aminopeptidase 2)

Membrane-bound Aminopeptidase P2 is a hydrolase specific for N-terminal imido bonds. It is widely expressed in many adult tissues with the highest levels in the kidney. \n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation, ELISA and Neutralization. Other applications not tested.\n\nRecommended Dilutions: \nWestern Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit is ~10ng/lane under non-reducing and reducing conditions.\nImmunoprecipitation: 25ug/ml immunoprecipitates recombinant human X-prolyl aminopeptidase from conditioned cell medium. \nDirect ELISA: 0.5-1.0ug/ml. The detection limit is ~1ng/well.\nNeutralization: Inhibits/neutralizes the enzymatic activity of recombinant human X-prolyl aminopeptidase. X1049-56A was preincubated with the enzyme at different molar ratios at RT for 30 minutes. The enzyme was then assayed with K(Abz)PP-pNA. 50% of the proteolytic activity was inhibited by X1049-56A at ~1.5ug/ml (IC50) under conditions in which the enzyme was present at 2ug/ml and the substrate concentration was 0.05mM. Considering the molecular masses of the enzyme (72kD) and the antibody (150kD), IC50 was achieved at ~1:2 molar ratio of the antibody to the enzyme. \nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

X1049-56A

Size

100ug

Applications

ELISA, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes human X-prolyl aminopeptidase. Does not crossreact with recombinant human XPNEP-1 or recombinant mouse XNEP-2. Species Sequence Homology: chimpanzee; 95%, canine; 84%, rat; 82%, mouse; 81%.