XAB2 (XPA Binding Protein 2, XPA-binding Protein 2, 0610041O14Rik, Adapter Protein ATH 55, ATH55, ATH-55, AV025587, Crn-related Protein Kim1, Crn Related Protein Kim1, DKFZp762C1015, HCNP, HCNP Protein, HCRN, KIAA1177, NTC90, RNA Splicing Factor, SYF1, SY

Tim50 is a component of the yeast Tim23 import machinery, which mediates translocation of presequence containing proteins across the mitochondrial inner membrane. By searching sequence databases, open reading frames encoding proteins with similarity to Tim50 and with a putative mitochondrial presequence were identified in the genomes of evolutionarily distant organisms, including human. Tim50 was found to interact with the N terminal intermembrane space domain of Tim23. Functional defects of Tim50 either by depletion of the protein or addition of anti Tim50 antibodies blocked the protein translocation across the inner membrane. A translocation intermediate accumulated at the translocator of the outer mitochondrial membrane (TOM) complex was cross linked to Tim50. Thus it can be concluded that Tim50, in cooperation with Tim23, facilitates transfer of the translocating protein from the TOM complex to the Tim23 complex.\n\nApplications: \nSuitable for use in ELISA (peptide) and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: A431 lysate.\n\nCellular Localization: Nuclear, detected in the splicing complex carrying pre-mRNA.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

X0801-01

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Nakatsu Y et al. XAB2, a novel tetratricopeptide repeat protein involved in transcription-coupled DNA repair and transcription. J Biol Chem 275:34931-7 (2000). <PUBMED:10944529>

Additional Info

Species Crossreactivity: Reacts with Human. Predicted to react with Mouse (100% identity with immunogen), Rat (100% identity with immunogen), canine and Zebrafish (83% identity with immunogen) due to sequence homology.