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XAF1, CT (XIAP-associated Factor 1, BIRC4-binding Protein, BIRC4BP, XIAPAF1)

XAF1, CT (XIAP-associated Factor 1, BIRC4-binding Protein, BIRC4BP, XIAPAF1)

Cat no: X1034-02G

Supplier: United States Biological
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XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Over-expression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death. XAF-1 is normally expressed in all adult and fetal tissues, but was found to be present in very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may play a critical role in maintaining survival of cancer cell lines. Both IFN-a2 and IFN-b can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human spleen cell lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: X1034-02G
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: ELISA, Immunohistochemistry, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Liston, P. et al. Nature Cell Biol. 3, 128 (2001). 2. Deveraux, QL. et al. Nature 388, 300 (1997). 3. Fong, WG. et al. Genomics 70, 113 (2000). 4. Leaman, DW. et al. J. Biol. Chem. 277, 28504 (2002).
Additional info: Recognizes human XAF-1. Species Crossreactivity: mouse.

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