Description:
5'-T^C T A G A-3'
3'-A G A T C^T-5'
Source:
Xanthomonas badrii
Supplied With:
R1625: Restriction Enzyme Buffer A, 10X
Concentration:
10u/ul
Unit Definition:
One unit is defined as the amount of XbaI required to digest 1ug of lambda DNA-dam-SmaI fragments in 1 hour at 30 degrees C in 50ul of assay buffer.
Diluent Buffer:
10mM Tris-HCl (pH 7.4 at 25 degrees C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
For longer periods, the Storage Buffer should be used.
Storage Buffer:
10mM Tris-HCl (pH 7.4 at 25 degrees C), 100mM KCl, 10mM 2-mercaptoethanol, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Storage and Stability:
For long-term storage, store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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