XBP1, CT (XBP1, TREB5, XBP2, X-box-binding protein 1, Tax-responsive element-binding protein 5) (Biotin)

This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.\n\nApplications:\nSuitable for use in Western Blot, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

043913-Biotin

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Biotin.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Sudarshan, S.R., et al. Biochem. Biophys. Res. Commun. 399(4):617-622(2010)\nThorpe, J.A., et al. Cell Stress Chaperones 15(5):497-508(2010)\nBagratuni, T., et al. Blood 116(2):250-253(2010)\nSawada, T., et al. J. Mol. Cell. Cardiol. 48(6):1280-1289(2010)\nLee, J.H., et al. Exp. Mol. Med. 42(5):386-394(2010)

Additional Info

Mouse

Alternative Names

XBP1, TREB5, XBP2, X-box-binding protein 1, Tax-responsive element-binding protein 5