XIAP-associated factor 1 (XAF1) binds to X-linked inhibitor of apoptosis (XIAP) and antagonizes the anticaspase activity of XIAP. XAF1 is expressed in all adult and fetal tissues but is frequently down-regulated in cancer cell lines. At least 7 isoforms of XAF1 are produced by alternative splicing.
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1ug/ml.
Immunohistochemistry (Paraffin and Formalin): 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.