XPG (Xeroderma pigmentosum Type G, ERCC5, ERCM2, Excision Repair Cross Complementing Rodent Repair Deficiency, UVDR, Xeroderma Pigmentosum Complementation Group G, XPGC)

Xeroderma pigmentosum (XP) is characterized high sensitivity to UV light and high incidence of skin cancer. XP fall into seven complementation groups, XPA to XPG. They are lacking a functional helicase, endonuclease, or lesion-recognizing proteins involved base excision repair. Xeroderma pigmentosum type G (XPG) is one of the rarest and phenotypically heterogeneous forms of genetic disorder. XPG gene encodes an acidic protein with an estimated MW ~133kD that confers normal UV-resistance. XPG is a member of the flap endonuclease 1 (FEN-1) structure-specific DNA repair endonuclease family responsible to UV-induced DNA repair. Human XPG makes the 3' incision during nucleotide excision repair of DNA. The enzyme cleaves model DNA bubble structures specifically near the junction of unpaired DNA with a duplex region. A 29aa region of human XPG (981-1009aa) contains the PCNA binding activity. A conserved Arg in XPG (Arg992) is crucial for its PCNA-binding activity. Replication protein A (RPA) binds specifically and directly to two excision repair proteins, Xeroderma pigmentosum damage recognition protein XPA and the endonuclease XPG.\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-5ug/ml. Detects > 185kD from DiFi and other cells and tissues.\nImmunoprecipitation: 5-10ug/mg of protein lysate.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

X1048-11

Size

50ug

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.1% sodium azide, 40% glycerol.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG2a,k

References

1. Cloud, K.G. et al., Mut. Res. 347: 55-60 (1995). 2. O'Donnovan, et al., J. Biol. Chem. 269: 15965-15968 (1994). 3. Aboussekhra, A., et al., Exp. Cell Res. 221: 326-332 (1995). 4. Vilpo, J.A., et al., Mol Cell. Biol. 15: 290-297 (1995). 5. Gary, R., et al., J. Biol. Chem. 272: 24522-24529 (1997). 6. Booulikas, T., et al., Anticancer res. 16: 693-708 (1996).

Additional Info

Recognizes human XPG.