XRCC1 (X-Ray Repair Cross Complementing Protein1)

X-Ray Repair Cross Complementing (XRCC1) plays a role in excision repair of DNA after ionizing irradiation. XRCC1 binds to DNA ligase III through the C-terminal 96 amino acids and to DNA polymerase beta through the N-terminal half.. In testis XRCC1 is expressed at high levels. Cells with a mutation of this gene exhibit decreased single strand break repair and reduced recombination repair. They show increased double strand breaks, and sister chromatid exchange is increased up to 10-fold. The XRCC1 might serve as a scaffold protein during base excision-repair. \n\nMW of Antigen: 85kD \n\nEpitope: Not determined\n\nApplications and Recommended Dilutions: \nImmunoprecipitation (Native verified) (Use Protein A) (Ab 2ug/mg protein lysate) \nWestern Blotting (Ab 1-2ug/ml for 2hrs at RT) \nImmunohistochemistry (Formalin/paraffin) (1-2ug/ml for 30 min at RT; is better) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator. \n\nPositive Control: Ls174T, HT29, or HeLa cells. Normal testis. \n\nCellular Localization: Nuclear\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20 degrees C or colder. Aliquots are stable for at least 12 months at-20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts