XRCC1 (X-Ray Repair Cross Complementing Protein1)

XRCC1 (X-ray cross complementing factor-1) is involved in DNA base excision repair. XRCC1 is a 70kDa protein that has been shown to be physically associated with other DNA repair enzymes, including poly (ADP-ribose) polymerase (PARP), DNA Ligase III and DNA polymerase beta. XRCC1 contains a BRCT domain (for BRCA1 carboxyl terminus) which was originally found in the tumor suppressor protein BRCA1. Recently, the BRCT domain has been shown to mediate the protein-protein interaction of XRCC1 and DNA Ligase III-a.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMCF7 and HBL100 cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

X9501-05

Size

50ul

Applications

ELISA, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a serum in 0.1% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1.Masson, M., et al. Mol. Cell. Biol. 18: 3563-3571, 1998.\n2.Zhang, X., et al. EMBO J. 17: 6404-6411, 1998.\n3.Taylor, R.M., et al. Curr. Biol. 8: 877-880, 1998.\n4.Cappelli, E., et al. J. Biol. Chem. 272: 23970-23975, 1997.\n5.Caldecott, K.W., et al. Nuc. Acids Res. 24: 4387-4394, 1996.\n

Additional Info

Recognizes human XRCC1.