XRCC3 (DNA Replication, Repair and Recombination Factor)

Five human RAD51 homologs have been identified: XRCC2, XRCC3, RAD51B, RAD51C, and RAD51D. Each of these homolgs interacts with one or more of the others, with all of the proteins involved in one complex or multiple smaller complexes. XRCC3 (x-ray cross complementing) was first identified as a human gene complementing the DNA damage sensitivity, chromosonal instability and impaired growth of the mutant hamster cell line irs1SF. This fact coupled with XRCC3 being a member of the RAD51 gene family have implied a role in the repair of DNA damage by homologous recombination.\n\nBy Western blot, this antibody recognizes a band at 38kDa, representing XRCC3. There are multiple bands for TK6 and HeLa cell extracts (this is normal and seen with XRCC3 recombinant protein). The band just above the XRCC3 band does not appear in nuclear extracts.\n\nSuggested Working Diultions:\nWestern Blot-1:5,000 (ECL, using 100 ug Hela lysate)\n\nPositive control: TK6 lysates and HeLa lysates, whole cell)

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SPECIFICATIONS

Catalog Number

X9503-01

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Schild, D., et al. Evidence for simultaneous protein interactions between human Rad51 paralogs. J. Biol. Chem. 275(22): 16443-9, 2000. 2. Brenneman, M.A., et al. XRCC3 is required for efficient repair of chromosone breaks by homolgous recombination. Mutation Research. 459(2): 89-97, 2000. 3. John Thacker. A surfeit of RAD51-like genes? Trends Genet. 15(5): 166-8, 1999.

Additional Info

Specific for XRCC3. No crossreactivity was observed with XRCC2, RAD51, RAD51B, RAD51C or RAD51D. Species crossreactivity: Reacts with human XRCC3.