XRCC3 (DNA Replication, Repair and Recombination Factor)

Five human RAD51 homologs have been identified: XRCC2, XRCC3, RAD51B, RAD51C, and RAD51D. Each of these homologs interacts with one or more of the others, with all of the proteins involved in one complex or multiple smaller complexes. XRCC3 (x-ray cross complementing) was first identified as a human gene complementing the DNA damage sensitivity, chromosonal instability and impaired growth of the mutant hamster cell line irs1SF. This fact coupled with XRCC3 being a member of the RAD51 gene family have implied a role in the repair of DNA damage by homologous recombination.\n\nFor Western Blotting, use at a dilution of 1:500 (ECL). A band of ~38kD should be detected.

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SPECIFICATIONS

Catalog Number

X9503-02

Size

100ul

Applications

WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as ascites

P Type

Mab

Purity

Ascities

Isotype

IgG1

References

1. Schild, D., et al. J. Biol. Chem. 275(22): 16443-9 (2000) 2. Brenneman, M.A., et al. Mutation Research. 459(2): 89-97 (2000) 3. John Thacker. Trends Genet. 15(5): 166-8 (1999)\nGeneral References:\n1. Masson, et al. Identification and purification of two distinct complexes containing the five RAD51 paralogs. Genes & Dev. 15: 3296-3307 (2001). 2. Masson, et al. Complex formation by the human RAD51C and XRCC3 recombination repair proteins. PNAS. 98: 8440-8446 (2001). 3. Tebbs, et al. Correction of chromosomal instability and sensitivity to diverse mutagens by a cloned cDNA of the XRCC3 DNA repair gene. PNAS. 92: 6354-6358

Additional Info

Specific for human XRCC3. It does not detect Rad51B, Rad51D, Rad51C, Rad51 or XRCC2 in Western blot analysis.