Y-27632 (ROCK Inhibitor)

Y-27632 (also called Y27632 or Y 27632) is a potent, cell permeable, selective and ATP-competitive Rho-associated protein kinases inhibitor, including p160ROCK, ROCK-II and PRK2 inhibitor. Ki values are 0.14, 26, 25 and >250uM for p160ROCK, PKC, cAMP-dependent protein kinase and myosin light-chain kinase, respectively. Prevents apoptosis as well as enhance the survival and cloning efficiency of dissociated human embryonic stem cells (hES) without affecting their pluripotency. Tumor cell invasion and metastasis suppressor. Smooth muscle relaxant. Decreases liver fibrosis by hepatic stellate cell growth inhibition. Antinociceptive. Blocks generation of inflammatory cytokines. Stem cell research modulator. Increases survival rate of hES cells undergoing cryopreservation.\n\nSolubility: \nWater, DMSO, methanol.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute, aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nImportant Note: \nThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written \nauthorization of United States Biological. \n\nToxicity and Hazards:\nAll products should be handled by qualified personnel only, trained in laboratory procedures.

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SPECIFICATIONS

Catalog Number

Y0110

Size

1mg

Form

Supplied as a white to off-white solid.

Purity

(same/more than) 98% (HPLC). Contains approx.1.4% water.

References

Product Reference: \n?Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension: M. Uehata, et al.; Nature 389, 990 (1997)\n?An essential part for Rho-associated kinase in the transcellular invasion of tumor cells: K. Itoh, et al.; Nat. Med. 5, 221 (1999)\n?Use and properties of ROCK-specific inhibitor Y-27632: S. Narumiya, et al.; Meth. Enzymol. 325, 273 (2000)\n?Specificity and mechanism of action of some commonly used protein kinase inhibitors: S.P. Davies, et al.; Biochem. J. 351, 95 (2000)\n?Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases: T. Ishizaki, et al.; Mol. Pharmacol. 57, 976 (2000)\n?Y-27632 potentiates relaxant effects of beta 2-adrenoceptor agonists in bovine tracheal smooth muscle: T. Nakahara, et al.; Eur. J. Pharmacol. 389, 103 (2000)\n?A p160ROCK-specific inhibitor, Y-27632, attenuates rat hepatic stellate cell growth: H. Iwamoto, et al.; J. Hepatol. 32, 762 (2000)\n?Inhibition of intrahepatic metastasis of human hepatocellular carcinoma by Rho-associated protein kinase inhibitor Y-27632: M. Takamura, et al.; Hepatology 33, 577 (2001)\n?Rho-kinase inhibitor, Y-27632, has an antinociceptive effect in mice: K. Buyukafsar, et al.; Eur. J. Pharmacol. 541, 49 (2006)\n?Novel Rho kinase inhibitors with anti-inflammatory and vasodilatory activities: C. Doe, et al.; JPET 320, 89 (2007)\n?A ROCK inhibitor permits survival of dissociated human embryonic stem cells: K. Watanabe, et al.; Nat. Biotechnol. 25, 681 (2007)\n?The ROCK inhibitor Y-27632 enhances the survival rate of human embryonic stem cells following cryopreservation: X. Li, et al.; Stem Cells Dev. 17, 1079 (2008)\n?Effect of Rho-associated kinase (ROCK) inhibitor Y-27632 on the post-thaw viability of cryopreserved human bone marrow-derived mesenchymal stem cells: B.C. Heng; Tissue Cell 41, 376 (2009)\n?Effect of ROCK inhibitor Y-27632 on normal and variant human embryonic stem cells (hESCs) in vitro: its benefits in hESC expansion: K. Gauthaman, et al.; Stem Cell Rev. 6, 86 (2010)\n?Pharmacological inhibition of Rho-kinase signaling with Y-27632 blocks melanoma tumor growth: A. Routhier, et al.; Oncol. Rep. 23, 861 (2010)\n?The ROCK inhibitor Y-27632 improves recovery of human embryonic stem cells after fluorescence-activated cell sorting with multiple cell surface markers: N. Emre, et al.; PLoS One 5, e12148 (2010)

Alternative Names

(R)-(+)-trans-4-(1-Aminoethyl)-N-(4-pyridyl)cyclohexanecarboxamide dihydrochloride