YAP1 (65 kDa Yes-associated Protein, YAP65, Yorkie Homolog)

Applications:\nSuitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 30ug/ml\nImmunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nAA Sequence:\nQIVHVRGDSETDLEALFNAVMNPKTANVPQTVPMRLRKLPDSFFKPPEPKSHSRQASTDAGTAGALTPQHVRAHSSPASLQLGAVSPGTLTPTGVVSGPAATPTAQHLR\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

135472

Size

100ug

Applications

ELISA, IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a,k

References

1. Tead4 is constitutively nuclear, while nuclear vs. cytoplasmic Yap distribution is regulated in preimplantation mouse embryos. Hirate Y, Cockburn K, Rossant J, Sasaki H.Proc Natl Acad Sci U S A. 2012 Dec 11;109(50):E3389-90. doi: 10.1073/pnas.1211810109. Epub 2012 Nov 20. 2. YAP1 Recruits c-Abl to Protect Angiomotin-Like 1 from Nedd4-Mediated Degradation. Skouloudaki K, Walz G.PLoS One. 2012;7(4):e35735. Epub 2012 Apr 27. 3. Hippo pathway regulation by cell morphology and stress fibers. Wada K, Itoga K, Okano T, Yonemura S, Sasaki H.Development. 2011 Sep;138(18):3907-14. Epub 2011 Aug 10. 4. Inactivation of Merlin in malignant mesothelioma cells and the Hippo signaling cascade dysregulation. Sekido Y.Pathol Int. 2011 Jun;61(6):331-44. doi: 10.1111/j.1440-1827.2011.02666.x. Epub 2011 May 2. 5. Identification of miR-193b targets in breast cancer cells and systems biological analysis of their functional impact. Leivonen SK, Rokka A, Ostling P, Kohonen P, Corthals GL, Kallioniemi O, Perala M.Mol Cell Proteomics. 2011 Apr 21. [Epub ahead of print] 6. LATS2 Is a Tumor Suppressor Gene of Malignant Mesothelioma. Murakami H, Mizuno T, Taniguchi T, Fujii M, Ishiguro F, Fukui T, Akatsuka S, Horio Y, Hida T, Kondo Y, Toyokuni S, Osada H, Sekido Y.Cancer Res. 2011 Feb 1;71(3):873-83. Epub 2011 Jan 18. 7. JNK phosphorylates Yes-associated protein (YAP) to regulate apoptosis. V Tomlinson, K Gudmundsdottir, P Luong, K-Y Leung, A Knebel, S Basu.Cell Death and Disease (2010) 1, e29; doi:10.1038/cddis.2010.7 8. Merlin Is a Potent Inhibitor of Glioma Growth. Lau YK, Murray LB, Houshmandi SS, Xu Y, Gutmann DH, Yu Q.Cancer Res. 2008 Jul 15;68(14):5733-42.

Additional Info

Recognizes human YAP1.