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YARS2, Recombinant, Human, aa17-477, His-Tag (Tyrosine--tRNA Ligase, Mitochondrial, Tyrosyl-tRNA Synthetase, TyrRS, CGI-04)

Cat no: 045472

YARS2, Recombinant, Human, aa17-477, His-Tag (Tyrosine--tRNA Ligase, Mitochondrial, Tyrosyl-tRNA Synthetase, TyrRS, CGI-04)

YARS2, also known as tyrosyl-tRNA synthetase, catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).\n\nSource:\nRecombinant corresponding to aa17-477 from human YARS2, fused to His-tag at N-terminus, expressed in E.coli.\n\nMolecular Weight:\n~53.7kD (482aa)\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MTLNLSVLLP LGLRKAHSGA QGLLAAQKAR GLFKDFFPET GTKIELPELF DRGTASFPQT IYCGFDPTAD SLHVGHLLAL LGLFHLQRAG HNVIALVGGA TARLGDPSGR TKEREALETE RVRANARALR LGLEALAANH QQLFTDGRSW GSFTVLDNSA WYQKQHLVDF LAAVGGHFRM GTLLSRQSVQ LRLKSPEGMS LAEFFYQVLQ AYDFYYLFQR YGCRVQLGGS DQLGNIMSGY EFINKLTGED VFGITVPLIT STTGAKLGKS AGNAVWLNRD KTSPFELYQF FVRQPDDSVE RYLKLFTFLP LPEIDHIMQL HVKEPERRGP QKRLAAEVTK LVHGREGLDS AKRCTQALYH SSIDALEVMS DQELKELFKE APFSEFFLDP GTSVLDTCRK ANAIPDGPRG YRMITEGGVS INHQQVTNPE SVLIVGQHIL KNGLSLLKIG KRNFYIIKWL QL\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

045472

Size

20ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 1mM DTT, 0.1M sodium chloride, 20% glycerol.

Purity

~85% (SDS-PAGE)

References

1. Bonnefond L., et al. (2005) Biochemistry. 44:4805-4816. 2. Riley L.G., et al. (2010) Am. J. Hum. Genet. 87:52-59.

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