YBOX1 (YB-1, YBX1)

Human DNA-binding proteins, dbpA and dbpB (YBOX-1), are members of a protein family containing a cold-shock domain, and are regarded as transcriptional regulators (1). The expression of HLA class II genes is regulated by a series of cis-acting elements and trans-acting factors. Several cis-acting elements have been identified and have been termed the Z box, X box, Y box, octamer, and "TATA" box. The Y box contains an inverted CCAAT box. An inverse correlation of YB-1 and HLA-DR beta chain mRNA levels suggests that YB-1 is a negative regulatory factor (2). Overexpression of YB-1 resulted in increased levels of PTP1B. Furthermore, depletion of YB-1 protein, by expression of a specific antisense construct, led to an approximately 70% decrease in expression of PTP1B, but no change in the level of its closest relative, TC-PTP. Data reveal an important role for YB-1 as a regulator of PTP1B expression, and further highlight PTP1B as a critical regulator of insulin- and cytokine-mediated signal transduction (3).\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation, Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:10,000-1:20,000 \nFlow cytometry: 1:60 \nImmunoprecipitation: 1:50 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

Y3051

Size

100ul

Applications

FC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50 mM Tris- Glycine, pH 7.4, 0.15 M sodium chloride, 0.01% sodium \nazide, 0.05% BSA and 40% Glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1.Kudo S, et al. Eur J Biochem 231(1):72-82, 1995 2.Didier DK, et al. Proc Natl Acad Sci 85(19):7322-6, 19883.Fukada T, et al. EMBO J 22(3):479-93, 2003

Additional Info

Recognizes human YBOX1.