Yeast Kex-2, Recombinant (Endoproteinase Lys/Arg-Arg)

Proteases (also called Proteolytic Enzymes, Peptidases, or Proteinases) are enzymes that hydrolyze the amide bonds within proteins or peptides. Most proteases act in a specific manner, hydrolyzing bonds at or adjacent to specific residues or a specific sequence of residues contained within the substrate protein or peptide. Proteases play an important role in most diseases and biological processes including prenatal and postnatal development, reproduction, signal transduction, the immune response, various autoimmune and degenerative diseases, and cancer. They are also an important research tool, frequently used in the analysis and production of proteins. Kex-2 cleaves at the carboxyl end of the recognition sequences Arg-Arg/X and Lys-Arg/X. Recombinant Yeast Kex-2 is a 59.9kD protease consisting of 554aa. Source: Recombinant protein corresponding to Yeast Kex-2, expressed in Hi-5 Insect cells. Biological Activity: Recombinant Kex-2 from High-5 insect cells contains the same specific activity and recognition sequence specificity as yeast derived KEX-2. 1 milligram of recombinant KEX-2 contains activity equivalent to 40 units of yeast derived KEX-2. Cleaves at the carboxyl side of K/R-R. Endotoxin: <0.1ng/ug (1EU/ug) AA Sequence: APPMDSSLLP VKEAEDKLSI NDPLFERQWH LVNPSFPGSD INVLDLWYNN ITGAGVVAAI VDDGLDYENE DLKDNFCAEG SWDFNDNTNL PKPRLSDDYH GTRCAGEIAA KKGNNFCGVG VGYNAKISGI RILSGDITTE DEAASLIYGL DVNDIYSCSW GPADDGRHLQ GPSDLVKKAL VKGVTEGRDS KGAIYVFASG NGGTRGDNCN YDGYTNSIYS ITIGAIDHKD LHPPYSEGCS AVMAVTYSSG SGEYIHSSDI NGRCSNSHGG TSAAAPLAAG VYTLLLEANP NLTWRDVQYL SILSAVGLEK NADGDWRDSA MGKKYSHRYG FGKIDAHKLI EMSKTWENVN AQTWFYLPTL YVSQSTNSTE ETLESVITIS EKSLQDANFK RIEHVTVTVD IDTEIRGTTT VDLISPAGII SNLGVVRPRD VSSEGFKDWT FMSVAHWGEN GVGDWKIKVK TTENGHRIDF HSWRLKLFGE SIDSSKTETF VFGNDKEEVE PAATESTVSQ YSASSTSISI SATSTSSISI GVETSAIPQT TTASTDPDSD PNTP Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.