Yes is the cellular homolog of the Yamaguchi sarcoma virus oncogene. This protein has tyrosine kinase activity and belongs to the src family of proteins. Yes is closely related to thymidylate synthase. It promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46.
Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Immunofluorescence: 1:100-1:200
Western Blot: 1:500-1:3000
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.