YMER (Chromosome 3 Open Reading Frame 6, C3orf6, Coiled-coil Domain-containing Protein 50, CCDC50, DFNA44, Protein Ymer)

YMER is a 306aa soluble cytoplasmic protein associated with microtubules of the cytoskeleton and mitotic apparatus. It has a coiled-coil domain and two motifs interacting with ubiquitin (MIUs). In response to EGF signaling, YMER undergoes tyrosine phosphorylation and ubiquitination and is also involved in the negative regulation of the EGF receptor. It has two isoforms, YMER-L and YMER-S, that are co-expressed in placenta, liver, lung, kidney and pancreas. However, YMER-S, the dominant YMER isoform expressed in human cells, is an inhibitor of EGFR downregulation. Defects in YMER leads to autosomal dominant non-syndromic sensorineural deafness type 44 (DFNA44). YMER shows ubiquitous expression in most of the human tissues.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.05-0.5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman, mouse, rat skeletal muscle\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

Y2065-15A

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, NHP

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Modamio-Hoybjor, S. et al. Am. J. Hum. Genet. 80:1076-1089 (2007). 2. Modamio-Hoybjor, S. et al. Hum. Genet. 112:24-28 (2003). 3. Tashiro, K. et al. J. Biol. Chem. 281:24612-24622 (2006). 4. Vazza, G. et al. Gene. 314:113-120 (2003).

Additional Info

Recognizes human YMER. Species Crossreactivity: chimpanzee, monkey. Species sequence homology: bovine, canine, rat.