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ZAP 70, aa326-341 (TCR Zeta Associated Protein kinase 70kD)

Cat no: Z0300

ZAP 70, aa326-341 (TCR Zeta Associated Protein kinase 70kD)

ZAP70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP70 is expressed exclusively in T cells and natural killer cells and is required for T cell receptor activation, which triggers an immune response. ZAP70 is activated when the protein tyrosine kinase Lck phosphorylates the tyrosine residues on ZAP70. Phosphorylation of ZAP70 is crucial to the recruitment of additional molecules, such as Vav, Ras, and PLC gamma-1, to the membrane-associated signaling complex and for the induction of downstream signaling events. Mutation of the ZAP70 genes results in a form of Severe Combined Immunode?ciency Syndrome (SCID) in humans. Recent evidence has suggested that ZAP70 should be considered as an effective biomarker in Chronic Lymphocytic Leukemia (CLL).\n\nApplications: Suitable for use in Western Blotting, Immunoprecipitation.\n\nRecommended Dilution:\nWestern Blotting: 1:1000 (detected p70 ZAP , which co-migrates with the 66kDa protein standard, in 20ug of a modified-RIPA lysate of human Jurkat cells. On immunoblots, this antiserum also recognizes two bands that migrate between the 66kDa and 97kDa protein standards. Recognition of these proteins is blocked by pre-incubating the antiserum with the immunizing peptide at 10mM). \n\nPositive Antigen Control: Jurkat cell lysate. Use 20ug per lane for minigels.\n\nImmunoprecipitation: 5ul

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SPECIFICATIONS

Catalog Number

Z0300

Size

150ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Neat serum

P Type

Pab

Purity

Neat serum

Isotype

IgG

References

Chan, Iwashima, Turck and Weiss, Cell, 73: 209, 1993. Chan, Irving, Fraser and Weiss, Proc. Natl. Acad. Sci. USA, 88: 9166, 1991.

Additional Info

Recognizes human ZAP-70. Species crossreactivity: mouse

Read more on Supplier website

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

More info

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

More info

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

More info
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