ZAP 70 (TCR Zeta Associated Protein kinase 70kD)

Mutations in ZAP-70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP-70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course.\n\nApplications:\nWestern Blot: (Ab 1-2ug/ml for 2hrs at RT)\nImmunohistology: (Formalin/paraffin) (Use antibody at 1:100 to 1:200 for 60 min at RT) Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.\n\nPositive Control: Tonsil, Jurkat cells, T- and NK-cells\n\nCellular Localization: Cytoplasmic (with occasional staining of macrophage lysozymes)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

Z0300-06C

Size

250ul

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride and 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

Crespo, M., et al, (2003). ZAP-70 expression as a surrogate for immunoglobulin-variable-region mutations in chronic lymphocytic leukemia. N Engl J Med, 348: 1764-75. (Flow Cytommetry) Silvin, C., et al, (2001). A role for Wiskott-Aldrich syndrome protein in T-cell receptor-mediated transcriptional activation independent of actin polymerization. J Biol Chem, 276: 21450-7. (WesternBlot) Wiestner, A., et al, (2003). ZAP-70 expression identifies a chronic lymphocytic leukemia subtype with unmutated immunoglobulin genes, inferior clinical outcome, and distinct gene expression profile. Blood, 101: 4944-51. (IHC)

Additional Info

Recognizes ZAP-70. Species Crossreactivity: Human.