ZAP 70 (Tyr319) Syk (Tyr352), phosphorylated (TCR Zeta Associated Protein kinase 70kD)

ZAP70, a Syk family protein tyrosine kinase expressed in T and NK cells, plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement. Following TCR engagement, ZAP70 is rapidly phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a transphosphorylation by the Src family tyrosine kinase Lck. Tyrosine phosphorylation of ZAP70 correlates with its increased kinase activity and couples to downstream signaling events. Phosphorylation of Tyr319 is required for the assembly of a ZAP70-containing signaling complex that leads to the activation of the PLC-gamma1-dependent and Ras-dependent signaling cascades in antigen-stimulated T cells. The orthologous Tyr352 residue in Syk is also involved in the association of PLC-gamma1. Phosphorylation of ZAP70 is crucial to the recruitment of additional molecules, such as Vav, Ras, and PLC gamma-1, to the membrane-associated signaling complex and for the induction of downstream signaling events. Mutation of the ZAP70 genes results in a form of Severe Combined Immunode?ciency Syndrome (SCID) in humans. Recent evidence has suggested that ZAP70 should be considered as an effective biomarker in Chronic Lymphocytic Leukemia (CLL).\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Flow Cytometry. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunocytochemistry (for IF): 1:100\nFlow Cytometry: 1:25\n\nOptimal dilutions to be determined by the researcher.\n\nMolecular Weight:\n70kD (Zap-70), 72kD (Syk)\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

Z0300-06

Size

100ul

Applications

ELISA, FC, ICC, IF, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Chu, D. H. et al. (1998) Immunol. Rev. 165, 167

Additional Info

Detects endogenous levels of human Zap-70 only when phosphorylated at Tyr319. Crossreacts with endogenous levels of Syk when phosphorylated at Tyr352. \nSpecies crossreactivity: Human and mouse based on 100% sequence homology.