ZAP 70 (Zeta Chain Associated Protein Kinase 70kD, Zeta Chain Associated Protein Kinase 70kDa, Zeta-chain Associated Protein Kinase 70kDa Isoform 1, Zeta-chain Associated Protein Kinase 70kDa Isoform 2, Zeta Chain TCR Associated Protein Kinase 70kD, Zeta-

ZAP70(zeta-chain associated protein kinase), a 70kD member of the SYK tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Zap70 controls TCR(T-cell antigen receptor)-linked signal transduction pathways. Its key role in thymocytes development and mature T lymphocytes activation has been illustrated by the characterization of several human immunodeficiencies presenting with mutations in the zap-70 gene. ZAP-70 is also expressed in several types of B-cell neoplasm and is easily detected by immunohistochemistry, providing a useful prognostic marker in patients with chronic lymphocytic leukemia.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry (Fr) (paraffin-embedded sections). Other applications not tested.\n\nRecommended Dilution:\nELISA 1: 10,000, \nWestern Blot 1: 500- 1: 2000 , \nImmunohistochemistry-Paraffin 1: 500- 1: 2000 \nImmunohistochemistry-Frozen 1: 500- 1: 2000 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

Z0300-01J

Size

100ul

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1.Sigal Gelkop , Gerrald D. Gish, Yael Babichev J Immunol. 2005 Dec 15;175(12):8123-32 \n2.Joaquim Carreras , Neus Villamor ,Lluis Colomo J Pathol. 2005 Mar;205(4):507-13 \n3.Claire Hivroz Med Sci (Paris). 2005 Feb;21(2):150-5

Additional Info

Species Crossreactivity: Human.