ZBP1, Recombinant, Human (Insulin-like Growth Factor 2 mRNA-binding Protein 1, IGF2 mRNA-binding Protein 1, IMP-1, Coding Region Determinant-binding Protein, CRD-BP, IGF-II mRNA-binding Protein 1, VICKZ Family Member 1, Zip Code-binding Protein 1, ZBP-1,

As Gram-negative bacterial isolates producing plasmid-mediated IMP-1 metallo-beta-lactamase usually demonstrate resistance to various broad-spectrum beta-lactams, including cephamycins and carbapenems, transmission and proliferation of these microorganisms in clinical settings could become a clinical threat in the near future.\n\nSource:\nRecombinant protein corresponding to human IMP-1, expressed in E. coli.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

149545

Size

25ug

Applications

ELISA, WB

Form

Supplied as a liquid in 20mM Tris-HCl, 150mM sodium chloride, 1mM EDTA, 5mM glutathione, 0.5mM GSSH, 10% glycerol.

Purity

~90% (Nickel NTA Affinity Chromatography)

References

1. Yamaguchi Y. (2005) Probing the role of Asp-120(81) of metallo-beta-lactamase (IMP-1) by site-directed mutagenesis, kinetic studies, and X-ray crystallography. J Biol Chem. 280(21): 20824-32.2. Nielsen J. (1999) A family of insulin-like growth factor II mRNA-binding proteins represses translation in late development. Mol Cell Biol. 19(2): 1262-70.