ZBP1 (Z-DNA binding protein 1, C20orf183, DAI, dJ718J7.3, DLM1, DLM-1, Tumor stroma and activated macrophage protein DLM-1)

yZ-DNA binding protein 1 (ZBP1) belongs to a family of proteins that contain the Za domain which binds specifically to left-handed Z-DNA and Z-RNA (1). ZBP1 was initially identified as a novel gene that was up-regulated in activated macrophages in mice bearing ascites tumors, suggesting that is may play a role in processes such as host response in neoplasia (2). More recent reports indicate that the cytosolic ZBP1 has can act like the toll-like receptor TLR9 by detecting cytosolic double-stranded (ds) DNA and trigger induction of type I interferon and other innate immune responses. It is thought that the binding of ZBP1 to dsDNA enhances its association with innate immune response proteins such as the IRF3 transcription factor and the serine/threonine kinase TBK1 (also known as NAK) (3). Multiple isoforms of ZBP1 are known to exist.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5

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SPECIFICATIONS

Catalog Number

Z0400-03

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Schwartz T, Behlke J, Lowenhaupt K, et al. Structure of the DLM-1-Z-DNA complex reveals a conserved family of Z-DNA- binding proteins. Nat. Struct. Biol. 2001; 8:761-5. 2. Fu Y, Comella N, Tognazzi K, et al. Cloning of DLM-1, a novel gene that is up-regulated in active macrophages, using RNA differential display. Gene 1999; 204:157-63. 3. Takaoda A, Wang Z, Choi MK, et al. DAI (DLM-1/ZBP1) is a cytosolic DNA sensor and an activator of innate immune response. Nature 2007; 448:501-5.

Additional Info

Recognizes human ZBP1. Species crossreactivity: Mouse and rat.