Human PLZF is a 74kD nuclear protein that belongs to the POK family of transcriptional repressors. It is a 673aa protein that contains an N-terminal BTB domain, followed by an acidic domain, a proline-rich region and a C-terminal zinc-finger domain. PLZF forms homodimers with RARa and LAZ3 with its zinc-finger region. Alternate splice forms exist which are tissue-specific and show a deletion of either the BTB domain, the acidic region, or the proline-rich region. Human PLZF shares 96%, 97%, 96% and 96% aa identity with rat, mouse, canine, and bovine PLZF, respectively.
Applications:
Suitable for use in Flow Cytometry, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 2.5ug/106 cells
Western Blot: 1ug/ml
Immunocytochemistry: 8-25ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Quick Search
Products 
