ZBTB16 (Zinc Finger And BTB Domain-containing Protein 16, Promyelocytic Leukemia Zinc Finger Protein, Zinc Finger Protein 145, Zinc Finger Protein PLZF, PLZF, ZNF145)

Human PLZF is a 74kD nuclear protein that belongs to the POK family of transcriptional repressors. It is a 673aa protein that contains an N-terminal BTB domain, followed by an acidic domain, a proline-rich region and a C-terminal zinc-finger domain. PLZF forms homodimers with RARa and LAZ3 with its zinc-finger region. Alternate splice forms exist which are tissue-specific and show a deletion of either the BTB domain, the acidic region, or the proline-rich region. Human PLZF shares 96%, 97%, 96% and 96% aa identity with rat, mouse, canine, and bovine PLZF, respectively.\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 2.5ug/106 cells\nWestern Blot: 1ug/ml\nImmunocytochemistry: 8-25ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

Z0744-35B

Size

100ug

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG2A

Additional Info

Recognizes human PLZF. Species sequence homology: rat (96%), mouse (97%), canine (96%), and bovine (96%). No cross-reactivity with recombinant human ZBTB38, ZNF24, ZNF143, ZNF206, ZNF281, or ZNF423.