ZCRB1 (Zinc Finger CCHC-type and RNA-binding Motif-containing Protein 1, U11/U12 Small Nuclear Ribonucleoprotein 31kD Protein, U11/U12 snRNP 31kD Protein, U11/U12-31K)

ZCRB1 is a nuclear protein first identified in a differential display screen involving morphine-dependence-related genes. Its expression is increased following morphine treatment and has been observed to be elevated in HepG2 cells. ZCRB1 contains a CCHC-type zing finger RNA-binding motif and can interact with the DBA-binding domain of the stem cell regulator C/EBP transcription factors. ZCRB1 was identified as one of the protein components of U11/U12 snRNPs, which are components of U12-type spliceosome and function as a molecular bridge connecting both ends of the intron, suggesting ZCRB1 may play a key role in U12-type splicing.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nRaji Cell Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146462

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Wang H, Gao MX, Li L, et al. Isolation, expression, and characterization of the human ZCRB1 gene mapped to 12q2. Genomics 2007; 89:59-69. 2. Trivedi AK, Bararia D, Christopeit M, et al. Proteomic identification of C/EBP-DBD multiprotein complex: JNK1 activates stem cell regulator C/EBPalpha by inhibiting its ubiquitination. Oncogene 2007; 1789-801. 3. Will CL, Schneider C, Hossbach M, et al. The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome. RNA 2004; 10:929-41.

Additional Info

Recognizes human ZCRB1. Species Crossreactivity: mouse and rat.