Zinc Finger E-box Binding Homeobox 1 (ZEB1, AREB6, BZP, NIL-2A, NIL-2-A, TCF8, ZEB, ZFHEP, ZFHX1A)

ZEB1 inhibits interleukin-2 (IL-2) gene expression. May be responsible for transcriptional repression of the IL-2 gene. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3 (PPCD3) [MIM:609141]. Posterior polymorphous corneal dystrophy (PPCD) is a rare disease involving metaplasia and overgrowth of corneal endothelial cells. In patients with PPCD, these cells manifest in an epithelial morphology and gene expression pattern, produce an aberrant basement membrane, and, sometimes, spread over the iris and nearby structures in a way that increases the risk for glaucoma.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Diltuinos:\nELISA: 1:32,000\nWestern Blot: 0.05-0.2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman Heart and Mouse Heart lysates\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 monthsat -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPeptide Blocking:\nCorresponding peptide is available for Peptide Blocking studies. See Z0469-75A-P. Antibody

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SPECIFICATIONS

Catalog Number

Z0469-75A

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Mouse

Form

Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

Park SM, Gaur AB, Lengyel E, Peter ME. The miR-200 family determines the epithelial phenotype of cancer cells by targeting the E-cadherin repressors ZEB1 and ZEB2. Genes Dev. 2008 Apr 22 (7): 894-907.

Additional Info

Recognizes human ZEB1 ar ~120kD. Species sequence homology: mouse, rat.