ZIP4 homolog (ZIP4H), also known as testis expressed sequence 11 (Tex11) is a 947 amino
acid (aa) X chromosome-encoded protein that is selectively expressed in primordial male germ cells. It associates with Nbs1 of the Mre11 protein complex which results in localization to areas of synaptonemal contact between meiotic chromosomes. During meiosis, ZIP4H promotes sister chromatid synapsis and crossover in response to programmed double stranded DNA breaks. ZIP4H deficiency leads to increased spermatocyte apoptosis and male sterility. Alternate splicing in mouse generates an additional isoform with a substitution (aa 691-707) and truncation (aa 708-947) at the C-terminus. Within aa 768-947, mouse ZIP4H shares 44% and 73% aa sequence identity with human and rat ZIP4H, respectively.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 0.5-1ug/ml. Lysates from human 293T cells and 293T cells transfected with rmZIP4H and rhZIP4H were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 0.5ug/ml rat anti-mouse ZIP4H.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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