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Zip4H (Tex11, Testis Expressed 11, Testis Expressed Sequence 11, TGC1, TSGA3)

Cat no: Z0545-01A

Zip4H (Tex11, Testis Expressed 11, Testis Expressed Sequence 11, TGC1, TSGA3)

Testis expressed sequence 11 (Tex11), also known as ZIP4 homolog, is a 947 aa X chromosome-encoded protein that is selectively expressed in primordial male germ cells. It associates with Nbs1 of the Mre11 protein complex which results in localization to areas of synaptonemal contact between meiotic chromosomes. During meiosis, ZIP4H/Tex11 promotes sister chromatid synapsis and crossover in response to programmed double stranded DNA breaks. ZIP4H/Tex11 deficiency leads to increased spermatocyte apoptosis and male sterility. Alternate splicing in mouse generates an additional isoform with a substitution (aa691-707) and truncation (aa708-947) at the C-terminus. Within aa768-947, mouse ZIP4H/Tex11 shares 44% and 73% aa sequence identity with human and rat ZIP4H/Tex11, respectively.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested. \n\nRecommended Dilutions:\nWestern Blot: 1ug/ml Detects ZIP4H protein from 293T cells transfected with the mouse ZIP4H gene with an approximate molecular weight of 110kD.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS, 0.02% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

Z0545-01A

Size

100ug

Applications

WB

Hosts

Goat

Reactivities

Mouse

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes mouse ZIP4H. Species sequence homology: human (44%) and rat (73%).

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