Death-associated protein kinase (DAPK3), which is a mediator of apoptosis, is induced by gamma interferon. DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities (1). Results suggest that DAPK3, in collaboration with Daxx and Par-4, mediates a novel nuclear pathway for apoptosis (2). It has been shown that the nuclear serine/threonine-specific kinase phosphorylates core histones H3 and H4, and myosine light chain in vitro. HeLa ZIPK phosphorylated the regulatory light chain of myosin II (MRLC) at both serine 19 and threonine 18 in a Ca2+/calmodulin independent manner. It interacts with transcription and splicing factors as well as with pro-apoptotic protein Par-4 suggesting that it participates in multiple cellular processes (3).
Applications:
Suitable for use in Western Blot, Flow Cytometry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:1000
Flow Cytometry: 1:10
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.