ZIPK (Zip Kinase, Death-Associated Protein Kinase 3, DAPK3, DLK, EC 2.7.11.1, FLJ36473)

Death-associated protein kinase (DAPK3), which is a mediator of apoptosis, is induced by gamma interferon. DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities (1). Results suggest that DAPK3, in collaboration with Daxx and Par-4, mediates a novel nuclear pathway for apoptosis (2). It has been shown that the nuclear serine/threonine-specific kinase phosphorylates core histones H3 and H4, and myosine light chain in vitro. HeLa ZIPK phosphorylated the regulatory light chain of myosin II (MRLC) at both serine 19 and threonine 18 in a Ca2+/calmodulin independent manner. It interacts with transcription and splicing factors as well as with pro-apoptotic protein Par-4 suggesting that it participates in multiple cellular processes (3).\n\nApplications: \nSuitable for use in Western Blot, Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nFlow Cytometry: 1:10\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

Z0550-25B

Size

100ul

Applications

FC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Kawai T, et al. Mol Cell Biol 18(3):1642-51, 1998. 2. Kawai T, et al. Mol Cell Biol 23(17):6174-86, 2003. 3. Preuss U, et al. Nucleic Acids Res 31(3):878-85, 2003. 4. Murata-Hori M, et al. FEBS Lett 451(1):81-4, 1999.

Additional Info

Recognizes Human Death-Associated Protein Kinase 3 (DAPK3).