DAPK3 (Death-associated protein kinase) is a Ca2+ calmodulin serine/threonine kinase which acts as a positive regulator of apoptosis through its catalytic activities. DAPK3 is a mediator of apoptosis and is induced by gamma interferon (1). Results suggest that DAPK3, in collaboration
with Daxx and Par-4, mediates a novel nuclear pathway for apoptosis (2). It has been shown that the nuclear serine/threonine-specific kinase phosphorylates core histones H3 and H4, and myosine light chain in vitro. DAPK3 interacts with transcription and splicing factors as well as with
pro-apoptotic protein Par-4 suggesting that it participates in multiple cellular processes (3).
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1:5000-1:10,000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.