ZMIZ1 (Zinc Finger MIZ Domain-containing Protein 1, PIAS-like Protein Zimp10, Retinoic Acid-induced Protein 17, KIAA1224, RAI17, ZIMP10, FLJ13541, hZIMP10, RP11-519K18.1, TRAFIP10)

ZIMP10, also known as ZMIZ1, is a novel PIAS (protein inhibitor of activated signal transducer and activator of transcription)-like protein initially identified as a transcriptional co-activator of the androgen receptor (AR). ZIMP10 and the related protein ZIMP7 interact with PIAS3 and enhances AR-mediated transcription. Later experiments showed that ZIMP10 is also a co-activator of the p53 tumor suppressor. Mice deficient in ZIMP10 result in embryonic lethality by E10.5; these embryos reveal severe defects in the reorganization of the yolk sac vascular plexus, indicating that ZIMP10 plays an important role in proper vascular development.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5ug/ml\nImmunocytochemistry: 10ug/ml\nImmunofluorescence: 20ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nK562 Cell Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146823

Size

100ug

Applications

ELISA, ICC, IF, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Sharma M, Li X, Wang Y, et al. hZimp10 is an androgen receptor co-activator and forms a complex with sUMO-1 at replication foci. EMBO J. 2003; 22:6101-14. 2. Beliakoff J and Sun Z. Zimp7 and Zimp10, two novel PIAS-like proteins, function as androgen receptor coregulators. Nucl. Recept. Signal. 2006; 4:e017. 3. Lee J, Beliakoff J, and Sun Z. The novel PIAS-like protein hZimp10 is a transcriptional co-activator of the p53 tumor suppressor. Nuc. Acids Res. 2007; 35:4523-34 4. Beliakoff J, Lee J, Ueno H, et al. The PIAS-like protein Zimp10 is essential for embryonic viability and proper vascular development. Mol. Cell. Biol. 2008; 28:282-92.

Additional Info

Recognizes human ZIMP10.