ZNF230 (Zinc Finger Protein 230, MGC8715, Ring Finger Protein 141, RNF141, ZFP26)

The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-DNA and protein-protein interactions. Abundant expression of this gene was found in the testicular tissue of fertile men, but was not detected in azoospermic patients. Studies of the mouse counterpart suggest that this gene may function as a testis specific transcription factor during spermatogenesis.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:16,000\nWestern Blot: 1ug/ml, observed in human testis lysate on ~25-30kD bands\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

Z0742-01C

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat, Bov, Can

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Zhang S, Qiu W, Wu H, Zhang G, Huang M, Xiao C, Yang J, Kamp C, Huang X, Huellen K, Yue Y, Pan A, Lebo R, Milunsky A, Vogt PH. The shorter zinc finger protein ZNF230 gene message is transcribed in fertile male testes and may be related to human spermatogenesis. Biochem J. 2001 Nov 1;359(Pt 3):721-7.

Additional Info

Recognizes human RNF141. Species sequence homology: Bovine, canine, mouse and rat.