Home  >  Products  >  ZNFN1A1 (DNA-binding Protein Ikaros, Hs.54452, hIk-1, IK1, IKAROS, Ikaros Family Zinc Finger Protein 1, IKZF1, LYF1, Lymphoid Transcription Factor LyF-1, PRO0758)
ZNFN1A1 (DNA-binding Protein Ikaros, Hs.54452, hIk-1, IK1, IKAROS, Ikaros Family Zinc Finger Protein 1, IKZF1, LYF1, Lymphoid Transcription Factor LyF-1, PRO0758)

ZNFN1A1 (DNA-binding Protein Ikaros, Hs.54452, hIk-1, IK1, IKAROS, Ikaros Family Zinc Finger Protein 1, IKZF1, LYF1, Lymphoid Transcription Factor LyF-1, PRO0758)

Cat no: Z0749-05

Supplier: United States Biological
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Ikaros family proteins, characterized by the presence of N-terminal zinc finger DNA-binding domain and C-terminal dimerization domain, belong to the Kruppel transcription factor superfamily. Ikaros family includes Ikaros, Aiolos, Helios, and possibly EOS and Pegasus (1). They can form homodimers as well as heterdimers with other members of the Ikaros family. Due to differential splicing, multiple isoforms can be generated and some behave in a dominant negative manner upon dimerization (2). Ikaros, also known as IKZF1 (Ikaros family zinc finger protein 1) and LYF-1 (Lymphoid transcription factor 1), is expressed abundantly in blood cells. Genetic studies in mice demonstrate that Ikaros is important for the normal development of B, T, natural killer, and dendritic cells, and that it functions as a tumor suppressor (3,4). Imbalanced expression of different Ikaros isoforms as well as deletions of Ikaros are associated with hematologic malignancies in humans (5-7). Applications: Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence (IF-IC): 1:100 Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: Z0749-05
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by Protein A affinity chromatography.
References: (1) Perdomo, J. et al. (2000) J Biol Chem 275, 38347-54. (2) Rebollo, A. and Schmitt, C. (2003) Immunol Cell Biol 81, 171-5. (3) Georgopoulos, K. et al. (1997) Annu Rev Immunol 15,155-76. (4) Nichogiannopoulou, A. et al. (1998) Semin Immunol 10, 119-25. (5) Tonnelle, C. et al. (2002) Leuk Lymphoma 43, 29-35. (6) Rebollo, A. and Schmitt, C. (2003) Immunol Cell Biol 81, 171-5. (7) Martinelli, G. et al. (2009) J Clin Oncol 27, 5202-7.
Additional info: Recognizes endogenous levels of total Ikaros. Species Crossreactivity: monkey, mouse, rat.

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