ZO-1 (Tight junction protein 1, Tight junction protein ZO-1, ZO1, ZO-1, Zona Occludens 1 Protein, Zonula Occludens 1 Protein)

Human zona occludens 1 (ZO-1), an ~220kD tight junction protein belonging to the membrane-associated guanlyate kinase (MAGUK) family. Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-1, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes. \n\nZO-1 is a peripheral membrane protein bound on the cytoplasmic surface of junctional contacts and is expressed in all tight junctions regardless of their properties. ZO-1 immunoprecipitates with its family member ZO-2. ZO-1 was shown to undergo tyrosine phosphorylation during tight junction formation and remodeling. Two different isoforms of ZO-1, alpha-minus and alpha-plus, have been described, which result from alternative splicing of an mRNA encoded by a single gene. The ZO-1 alpha-plus contains an 80 amino acids motif called alpha which is not present in ZO-1 alpha-minus. The alpha-containing isoform is found in most epithelial cell junctions. The short isoform (ZO-1 alpha-minus) is found both in endothelial cells and the highly specialized \nepithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. This difference in distribution provides molecular distinction among tight junctions. \n\nApplications:\nSuitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:50\nWestern Blot: 1:50\nImmunohistochemistry (frozen): 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

Z0750-02E

Size

100ug

Applications

IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Can

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

References

1. Balda, M et al; Two classes of tight junctions are revealed by ZO-1 isoforms. Am J Physiol 1993, 264: C918. 2. Pelletier, R et al; Differential distribution of the tight-junction-associated protein ZO-1 isoforms a-plus and a-minus in guinea pig Sertoli cells: a possible association with F-actin and G-actin. Biol Reprod 1997, 57: 367.

Additional Info

Recognizes both ZO-1 alpha-minus and alpha-plus human zona occludens 1 (ZO-1). Species crossreactivity: mouse, dog

Alternative Names

TJP1