ZO-2, CT (Tight Junction-associated Polypeptide, Zonula Occludens 2)

Tight junctions are intercellular junctions that act as both the barrier (or gate), and the fence. The barrier function of tight junctions regulates the passage of ions, water, and various macromolecules through paracellular spaces. The fence function maintains cell polarity, thus, tight junctions work as a fence to prevent intermixing of molecules in the apical membrane with those in the lateral membrane. Tight junctions are comprised of the membrane proteins ZO, occludin, claudins, and JAMs. The zonula occludens proteins, ZO-1, ZO-2 and ZO-3 are tight junction proteins that belong to the membrane-associated guanylated kinase (MAGUK) family. ZOs are involved in the organization of epithelial and endothelial intercellular junctions and bind to the cytoplasmic C-termini of junctional transmembrane proteins linking them to the actin cytoskeleton. ZOs contain some unique motifs not shared by other MAGUK family members, including nuclear localization and nuclear export signals and a leucine zipper-like sequence. ZOs also have potential involvement in cell growth and proliferation.\n\nApplications:\nSuitable for use in Western Blot and Immunofluorescence. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot: 1-3ug/ml\nImmunofluorescence: 2.5ug/ml. Cells need to be fixed with ice-cold ethanol for 30 minutes on ice prior to assays.\nOptimal dilutions to be determined by the researcher.\n\nPositive Controls:\nWestern Blot: CaCO2 human colorectal adenocarcinoma, MDCK II canine kidney, KNRK rat kidney, and TMCK-1 mouse kidney cell lysates..\nImmunofluorescence: CaCO2, MDCK II, and NRK52E.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

Z0760-01C

Size

100ug

Applications

IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Can

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Sawada N, et al. Med Electron Microsc 36(3): 147-56, 2003. 2. Traweger A, et al. J Biol Chem 278(4): 2692-700, 2003.

Additional Info

Recognizes the ~130-160kD human ZO-2 protein, isoforms A or C. A band of unknown origin lower than 75kD may be observed in certain lysates. Species Crossreactivity: rat, canine and mouse