ZO-2 (Tight Junction-associated Polypeptide, Zonula Occludens 2)

The ZO-1 and ZO-2 proteins share significant sequence homology to each other (51% identity), as well as to several other proteins which include the lethal(1)discs-large(dlg) tumor supressor gene product (dlg-A) of Drosophila, erythrocyte membrane-associated p55 protein, and PSSD-95/SAP90. These proteins share several conserved domains and are collectively referred to as the MAGUK family. The regions of homology among this family of proteins include a domain homologous to guanylate kinase (GUK), a single SH3 domain, and a variable number of N-terminal repeated domains termed PDZ domains. The ZO-1 and ZO-2 proteins are distinct from the other family member in that they possess C-terminal acidic and proline rich regions, and alternatively spliced domains at their C-termini. Although, ZO-1 and ZO-2 are structurally similar and display significant sequence homology, the proteins are divergent at their C-termini. This suggests that the C-terminal ends of ZO-1 and ZO-2 have distinct functions. The canine ZO-2 protein has a predicted molecular mass of ~132kD. However, the protein migrates with a mass of 160kD when analyzed by SDS-PAGE.\n\nApplications: \nSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 0.1-1ug/ml \nWestern Blot: 1-4ug/ml\nImmunohistochemistry (Mouse liver tissues): 4-5ug/ml\nNote: Weak fixation of tissues with 2% paraformaldehyde/picric acid followed by NO post-fixation is required to produce staining.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHeLa and canine MDCK cell lysates and mouse liver homogenates.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

Z0760

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Can

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1.

Additional Info

Specific for the canine ZO-2 protein (~160kD). Does exhibit crossreactivity with a few proteins of unknown identity in the 50-85kD region. Does not appear to crossreact with the related ZO-1 protein. Species crossreactivity: human and mouse.