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ZO-2 (Tight Junction-associated Polypeptide, Zonula Occludens 2)

Cat no: Z0760-01

ZO-2 (Tight Junction-associated Polypeptide, Zonula Occludens 2)

The zonula occludens proteins (ZOPs), ZO-1, ZO-2 and ZO-3 are tight-junction phosphorylated proteins that belong to the membrane-associated guanylated kinase (MAGUK) family. ZOs are involved in the organization of epithelial and endothelial intercellular junctions and bind to the cytoplasmic C-termini of junctional transmembrane proteins linking them to the actin\ncytoskeleton, ZOPs contain three PDZ domains, one SH3 domain, and a guanylate kinase-like domain, indicating that ZOPs may serve multiple purposes. ZOPs contain some unique motifs not shared by other MAGUK family members, including nuclear localization and nuclear export signals and a leucine zipper-like sequence. Their potential involvement in cell growing and proliferation has been suggested based on the observation that the N-terminal half of ZOPs displays a significant similarity to the product of the Drosophila tumor suppressor gene lethal disc-large (dlg).\n\nApplications:\nSuitable for use in Immunoprecipitation, Western Blot, Immunofluorescence and ELISA. Other applications have not been tested.\n\nRecommended Dilution:\nImmunoprecipitation (Native): 5-10ug\nWestern Blot: 1-3ug/ml\nImmunofluorescence: 1-3ug/ml\nELISA (Native): 0.1-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Controls:\nHuman CaCo2 and Canine MDCK cell lysates\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

Z0760-01

Size

100ug

Applications

ELISA, IF, IP, WB

Hosts

Mouse

Reactivities

Hum, Can

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1,k

References

1. Beatch M, et al. J of Biol Chem 271(42): 25723

Additional Info

Recognizes human ZO-2 at ~160kD. Species Crossreactivity: canine.

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