ZO-2 (Tight Junction-associated Polypeptide, Zonula Occludens 2)

Human zona occludens 2 (ZO-2), an ~160kD tight junction protein belonging to the membrane-associated guanlyate kinase (MAGUK) family. Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-2, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes. \n\nZO-2 can interact with zona occludens 1 (ZO-1). Furthermore, the PDZ2 domain of ZO-2 was shown to interact with connexin-43, the predominant connexin in epithelial and most other tissues which is involved in cell growth control and embryonic development. \n\nApplications:\nSuitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:50\nWestern Blot: 1:50\nImmunohistochemistry (frozen): 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

Z0760-01D

Size

100ug

Applications

IF, IHC, WB

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified

Additional Info

Recognizes human zona occludens 2 (ZO-2), an ~160kD tight junction protein belonging \nto the membrane-associated guanlyate kinase (MAGUK) family. Species crossreactivity: mouse

Alternative Names

TJP2