ZONA OCCLUDENS 1 alpha-plus (ZO-1-alpha-plus)

Zona Occludens 1 (ZO-1) is an ~220kD tight junction protein belonging to the membrane-associated guanlyate kinase family. Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO1 (PDZ) domain, a Src homology 3 (SH3) domain and an enzymatically inactive guanylate kinase domain. PDZ domains are 90aa protein- protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain- containing proteins, like ZO-1, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions and desmosomes. ZO-1 is a peripheral membrane protein bound on the cytoplasmic surface of junctional contacts and is expressed in all tight junctions regardless of their properties. ZO-1 immunoprecipitates with its family member ZO-2. ZO-1 was shown to undergo tyrosine phosphorylation during tight junction formation and remodeling. Two different isoforms of ZO-1, alpha-minus and alpha-plus, have been described, which result from alternative splicing of an mRNA encoded by a single gene. The ZO-1 alpha-plus contains an 80aa motif called alpha which is not present in ZO-1 alpha-minus. The alpha-containing isoform is found in most epithelial cell junctions. The short isoform (ZO-1 alpha-minus) is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. This difference in distribution provides molecular distinction among tight junctions.\nApplications:\nSuitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.\nRecommended Dilutions:\nImmunofluorescence: 1:50\nFlow Cytometry: 1:50\nWestern Blot: 1:50\nImmunohistochemistry (Frozen, paraffin): 1:50\nOptimal dilutions to be determined by the researcher.\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

Z0750-11

Size

100ug

Applications

FC, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Can

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Willott, E et al; Localization and differential expression of two isoforms of the tight junction protein ZO-1. Am J Physiol 1992, 262: C1119. 2. Balda, M et al; Two classes of tight junctions are revealed by ZO-1 isoforms. Am J Physiol 1993, 264: C918. 3. Pelletier, R et al; Differential distribution of the tight-junction-associated protein ZO-1 isoforms alpja-plus and alpha-plus in guinea pig Sertoli cells: a possible association with F-actin and G-actin. Biol Reprod 1997, 57: 367. 4. Van Itallie, C et al; Epidermal growth factor induces tyrosine phosphorylation and reorganization of the tight junction protein ZO-1 in A431 cells. J Cell Sci 1995, 108: 1735. 5. Kurihara, H et al; Diversity among tight junctions in rat kidney: glomerular slit diaphragms and endothelial junctions express only one isoform of the tight junction protein ZO-1. Proc Natl Acad USA 1992, 89: 7075.

Additional Info

Recognizes human ZONA OCCLUDENS 1 alpha-plus. \nSpecies Crossreactivity: mouse, rat and canine

Alternative Names

TJP1