Zonulin

Zonulin is a protein that participates in tight junctions between cells of the wall of the digestive tract. Initially discovered in 2000 as the target of zonula occludens toxin, secreted by cholera pathogen Vibrio cholerae, [1] it has been implicated in the pathogenesis of celiac disease [2] and diabetes mellitus type 1 [3]. It is being studied as a target for vaccine adjuvants [4]. ALBA Therapeutics is developing a zonulin receptor antagonist, AT-1001, that is currently in phase 2 clinical trials.\n\nApplications: \nSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:10,000\nImmunohistochemistry: Frozen sections\nImmunohistochemistry (Parffin): 1:300\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

Z0805

Size

20ul

Applications

ELISA, IHC

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder. Reconstitute with 20ul sterile ddH2O.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Skardelly M, Armbruster FP, Meixensberger J, Hilbig H, . Expression of Zonulin, c- kit, and Glial Fibrillary Acidic Protein in Human Gliomas. Translational Oncology 2. Wang W, Uzzau S, Goldblum SE, Fasano A. Human zonulin, a potential modulator of intestinal tight junctions. J Cell Sci 2000;113 Pt 24:4435-40. PMID 11082037. 3. Thomas KE, Sapone A, Fasano A, Vogel SN. Gliadin stimulation of murine macrophage inflammatory gene expression and intestinal permeability are MyD88-dependent: role of the innate immune response in Celiac disease. J Immunol 2006;176:2512-21. PMID 16456012. 4. Sapone A, de Magistris L, Pietzak M, Clemente MG, Tripathi A, Cucca F, Lampis R, Kryszak D, Carteni M, Generoed with increased gut permeability in subjects with type 1 diabetes and their relatives. Diabetes 2006;55:1443-9. PMID 16644703. 5. De Magistris MT. Zonula occludens toxin as a new promising adjuvant for mucosal vaccines. Vaccine 2006;24 Suppl 2:S2-60-1. PMID 16823929.

Additional Info

Recognizes human Zonulin