Zymolyase 20T Concentrate 50mg/ml, 0.1M Sorbitol (Lyticase, Yeast Lytic Enzyme)

Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is * Lyticase is similar but testing has shown that Zymolyase exhibits greater lytic activity.\n\nSee publication by United States Biological titled: A Comparison of Zymolyase, Lyticase and Glusulase.\n\nZymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below.\n\nZymolyase 20T Stock Concentrate 50mg/ml is a convenient preparation.\n\nApplications:\nProtoplast/Spheroplast Preparation\nYeast Cell Fusion\nYeast Cell Transformation\n\nAppearance: Pale yellow to brown solution/suspension\nActivity: (same/more than) 20u/mg\n\nPurity: Purified by ammonium sulfate precipitation.\n\nUnit Definition:\nOne unit of lytic activity is defined as that amount which indicates 30% of decrease in absorbance at 800nm (A800). Refer to Assay for Enzymatic Activity.\n\nWater: Molecular Biology Grade, RNase, DNase-Free\n\nFilter: 0.2um validated\n\nSorbitol: (same/more than) 98%\n\nSynonyms: Lyticase*, Yeast Lytic Enzyme\n\nSource: Arthrobacter luteus\n\nEssential Enzyme: b-1,3-glucan laminaripentaohydrolase\n\nOther activities contained:\nb-1,3-glucanase: ~1.5x10e3u/mg\nProtease: ~15u/mg\nMannanase: ~1.5x10e3u/mg\nAmylase, xylanase, phosphatase: Minute amounts\nDNase, Rnase: Trace levels reported\n(See reference No. 3 as to the definition of each enzyme unit. Each activity varies more of less among lots.)\n\nOptimum pH:\nFor cell wall lysis: 7.5, 35 degrees C \nFor yeast glucan hydrolysis: 6.5, 45 degrees C\n\nStable pH: 5-10\n\nHeat Stability: The lytic activity is lost on incubation at 60 degrees C for 5 minutes.\n\nProperties of Zymolyase:\nSpecificity (lytic spectrum)(5): Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloekera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomcopsis, Saccharomycodes, Schwanniomyces, etc\nSusceptible strains in low concentration (0.2 units/ml):\nAshbya, Endomyces, Kloekera, Kluyveromyces, Pullularia, Saccharomyces\nSusceptible strains in high concentration (2.0 units/ml):\nCandida, Debaryomyces, Eremothecium, Hansenula, Hanseniaspora, Lipomyces, Metschnikowia, Saccharomycopsis, Saccharomycodes, Schizosaccharomyces, Selenozyma, Trigonopsis, Wickerhamia\nSusceptibility depending upon strains:\nBretanomyces, Cryptococcus, Nadsonia, Picia, Rodosporidium, Schwanniomyces, Stephnoascus, Torulopsis\nNo susceptible strains:\nBullera, Pityosporum, Rhosotorula, Sporidiobolus, Sporobolomyces, Stetigmatomyces, Trichosporon\n\nActivators: SH compound such as cystein, 2-mercaptoethanol of dithiothreitol\n\nStorage and Stability: Stable for 12 months at -20C.\n\nCompanion Products:\nZ1000: Zymolyase 20T\nZ1001: Zymolyase 20T Concentrate 50mg/ml, 0.1M Sorbitol\nZymolyase 20T shows 20u/mg of the lytic activity, defined after, toward brewer's yeast cells (Saccharomyces cerevisiae, resting stage) or toward yeast cells of Saccharomyces cerevisiae IFO 0565 cultured statically in malt extract medium (Malt extract 2g, peptone 0.5g, water 100ml) at 20C for 34 hours.\n\nZ1004: Zymolyase 100T\nZ1005: Zymolyase 100T Concentrate 10mg/ml, 0.1M Sorbitol\nZymolyase 100T, further purified, whose specific activity is 100u/mg.\n\nNote: Zymolyase100T is less soluble than 20T and may not be completely dissolved in buffers. If this is the case use as a suspension.

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