Zyxin (ZYX, ZYX Protein, Zyxin 2, Zyxin-2, ESP-2, HED-2)

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.\n\nApplications: \nSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nImmunofluorescence (IC): 1:100\nWestern Blot: 1:100-2000 using whole extract \nof human cervix epitheloid carcinoma (HeLa) cells and a chemiluminescent detection reagent or using whole extract \nof mouse NIH-3T3 cells and a chemiluminescent detection reagent\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

Z9800-02

Size

100ul

Applications

IF, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Beckerle MC Zyxin: zinc fingers at sites of cell adhesion. Bioessays. 19:949-57 (1997). 2. Nix DA and Beckerle MC. Nuclear-cytoplasmic shuttling of the focal contact protein, zyxin: a potential mechanism for communication between sites of cell adhesion and the nucleus. J Cell Biol. 138:1139-47 (1997). 3. Macalma T et al. Molecular characterization of human zyxin. J Biol Chem. 271:31470-8 (1996). 4. Crawford AW & Beckerle MC Purification and characterization of zyxin, an 82,000-dalton component of adherens junctions. J Biol Chem 266:5847-53 (1991).

Additional Info

Recognizes human Zyxin. Species crossreactivity: mouse and rat.