Cloning

Looking for reliable and cost effective Cloning products for your research?

Cloning, or copying DNA sequences and inserting them into a vector, such as a plasmid or E. coli bacteria, in order to transfect that DNA into a cell or organism, plays an important role in many types of biological laboratory research. Cloning individual genes or other DNA fragments is a much less daunting process than reproductive cloning of an entire multicellular organism, such as a sheep; it is...

...used on a regular basis in clinical and research labs for synthesizing genes, amplifying DNA, and testing new therapies for genetic disorders.

Laboratory materials for genetic cloning include species-specific PCR primers for RT-PCR (reverse transcription followed by PCR) analysis of mRNA expression; tissue panels and matched pairs of tissue specific or universal cDNA (complementary DNA) from human adult normal and diseased tissues, human fetal tissues, rat, mouse, chicken, canine, monkey and plant tissues; species-specific or tissue-specific probes or oligonucleotides (DNA fragments); amplification, assay and RNA synthesis kits; ligands; cell culture media and albumin; transfection reagents; and primers and adapters.

Equipment for cloning research includes thermal cyclers for high-speed PCR; cooling blocks for incubating samples; hybridization incubators; cryogenic and ultralow freezers; microplate readers; autoclaves; centrifuges; homogenizers; water bath shakers; and DNA and RNA extractors/purifiers.

Additional cloning research aids include DNA and protein sequence analysis software; LIMS software; clone injection services; DNA sequencing services; and strains of E. coli for cloning or subcloning.

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