Categories Protein Biochemistry
Peptides are abundant in living organisms, actively participating in key biological processes such as hormone regulation, enzyme catalysis, and immune...
High-performance Liquid Chromatography (HPLC) is a modern chromatographic separation technique extensively utilized in biological, chemical, and medical...
The primary structure of proteins is fundamental to the biological function of proteins, and determining the primary structure aids in studying the function...
Protein isoform analysis is a method to study the differences in structure and function of proteins. Proteins are key molecules in life activities, and their...
In scientific research, there are two main types of analysis: qualitative and quantitative analysis. Between qualitative and quantitative analysis, there is...
Data-independent acquisition (DIA) is a non-dependent data scanning mode, a holographic mass spectrometry data acquisition mode based on the electrostatic...
Relative quantification of proteins is a MS-based technique that compares the relative proteins expression levels between different samples, analyzing...
Two-dimensional difference gel electrophoresis (2D-DIGE) is a novel quantitative proteomics technique that evolved from traditional two-dimensional gel...
STRING is a database that records predicted and experimentally validated protein-protein interactions (PPIs) across multiple species, including direct...
Proteomics mass spectrometry data is fundamental to proteomics analysis. The quality of the mass spectra is closely related to the accuracy of the retrieval...
KEGG (Kyoto Encyclopedia of Genes and Genomes) database is a database used for systematic analysis of metabolic pathways and functions of gene products in...
4D-DIA quantitative proteomics is an emerging high-throughput mass spectrometry technology that combines data-independent acquisition (DIA) strategies with...
The process where proteins separated by electrophoresis are transferred or 'blotted' from the gel matrix onto a membrane (usually nitrocellulose or...
AQUA absolute quantitative analysis is a targeted quantitative proteomics technique widely used in various quantitative proteomics studies. The absolute...
The label transfer method, in conjunction with crosslinking, facilitates the study of protein interactions by marking proteins that bind to target proteins....
In the biological activities of organisms, the function of proteins is achieved through interactions between proteins. Therefore, studying new protein...
Proteins exhibit asymmetric secondary structures such as ?-helices, ?-sheets, ?-turns, and others, leading to differential absorption of left and right...
Protein secondary structure analysis refers to the study of local spatial structures between continuous amino acid residues in proteins, mainly including...
High-performance liquid chromatography (HPLC) is a technique that utilizes chemical and physical interactions between the stationary phase (particles inside...
Far-western blot is a molecular biology method based on western blot technology that can be used to detect protein-protein interactions in vitro, especially...
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