11(beta)-Prostaglandin F2(alpha) EIA Kit

11(beta)-PGF2(alpha) is the primary plasma metabolite of PGD2 in vivo, the levels of which can increase from 6 pg/ml in a normal healthy volunteer to 490 ng/ml in patients with systemic mastocytosis. The normal human urinary excretion of 11(beta)-PGF2(alpha) is about 11 ng/mmol creatinine (~400 ng/24 hr), which is increased nearly 3-fold upon allergen-induced bronchoconstriction in asthmatics. Cayman's 11(beta)-PGF2(alpha) Assay is a competitive assay that provides accurate measurements of 11(beta)-PGF2(alpha) within the range of 7.8-1,000 pg/ml.

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SPECIFICATIONS

Catalog Number

516521

Form

96 Well

P Type

Assay Kits|Prostaglandins|Eicosanoids

Weight

0

Storage Temp

-20

Shipping Temp

-20

Additional Info

Prostaglandin D2 (PGD2) is the major eicosanoid released from stimulated mast cells. PGD2 is an unstable compound which is rapidly metabolized and eliminated from the circulation. This makes quantitation of PGD2 an unreliable indicator of its in vivo production. 11.beta.-PGF2.alpha. is the primary plasma metabolite of PGD2 in vivo, the levels of which can increase from 6 pg/ml in a normal healthy volunteer to 490 ng/ml in patients with systemic mastocytosis. The normal human urinary excretion of 11.beta.-PGF2.alpha. is about 11 ng/mmol creatinine (~400 ng/24 hr), which is increased nearly 3-fold upon allergen-induced bronchoconstriction in asthmatics. Unlike most prostaglandin metabolites, 11.beta.-PGF2.alpha. retains potent biological activity. 11-.beta.-PGF2.alpha. is equipotent to PGF2.alpha. in inducing human bronchial smooth muscle contractions and inhibition of adipose differentiation. 11.beta.-PGF2.alpha. was also shown to inhibit ADP or thrombin-induced human platelet aggregation at concentrations of 0.14 to 2.8 microM. The Cayman 11.beta.-PGF2.alpha. Assay is a competitive assay that provides accurate measurements of 11.beta.-PGF2.alpha. within the range of 7.8-1,000 pg/ml. Inter and intra-assay CV's of less than 15% can be achieved at most concentrations. This assay allows sensitive detection of 11.beta.-PGF2.alpha. in the most common sample matrix, which is urine. Plasma concentrations of 11.beta.-PGF2.alpha. are generally below the detection limit. For plasma and other complex sample matrices, we recommend purification of the sample prior to analysis; a purification protocol is included in the kit booklet. Measurements of 11.beta.-PGF2.alpha. in urine using our EIA gives values in the same range, but slightly higher than those obtained by GC-MS. This appears to be due to measurement of 11.beta.-PGF2.alpha. plus the 2,3-dinor metabolite of 11.beta.-PGF2.alpha..