2-Thiobarbituric Acid Reactive Substances (TBARS) Colorimetric BioAssay(TM) Kit

The TBARS assay was developed for the measurement of MDA production via lipid peroxidation and is a well-established and widely used method for assessment of oxidative damage. However, as typically performed, the traditional method for TBARS analysis is often criticized for lack of specificity and sensitivity. Indeed, currently-available TBARS assays require boiling the sample with strong acid which also generates interfering chromophores.\n\nThis assay uses a room temperature TBARS method that eliminates the requirement for a high concentration of a strong acid and cuts reaction time in half. By running the assay at room temperature, low levels of interfering substances are generated. This results in more accurate, precise and reliable TBARS measurements.\n\nAdvantages of this BioAssay(TM) Kit:\nIndustry leading sensitivity (250nM) \nEnhanced MDA measurement with use of proprietary reagent formulation \nReduced background signal\nShorter assay times; 15 minutes at RT\nUseful with any biological, nutraceutical or food sample\nLess expensive than other methods now on the market\nEliminates the requirement of boiling samples\nCan use either cuvette or microplate formats\n\nPrinciple of the Procedure:\nThis assay is based on the reaction of a chromogenic reagent, 2-thiobarbituric acid, with MDA at 25 degrees C. One molecule of MDA reacts with 2 molecules of 2-thiobarbituric acid via a Knoevenagel-type condensation to yield a chromophore with absorbance maximum at 532nm, as shown in Figure 1.\n\nKit Components:\n1. Indicator, 2-Thiobarbituric Acid, 2x1g\n2. Acid Reagent, 10% Acid Solution in Dimethylsulfoxide, 1x40ml\n3. MDA Standard, 10mM Malonaldehyde Tetrabutylammonium Salt, 1x100ul\n4. Microtiter plate, 2x96 wells\n\nStorage and Stability:\nStore components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T0700-89

Size

40Tests

References

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